A general method to select for M13 clones carrying base pair substitution mutants constructedin vitro
نویسندگان
چکیده
منابع مشابه
A general method to select for M13 clones carrying base pair substitution mutants constructed in vitro.
In this paper we describe a method to select base pair substitution mutants constructed in vitro. The mutagenesis is performed by forcing mistakes during in vitro synthesis from a primer annealed to a single stranded DNA template. The selection is based on the fact that, following transformation, the progeny of the strand elongated in vitro and the template strand have different phenotypes. The...
متن کاملConstruction of a Synthetic Vector for Preparation of a 100 Base Pair DNA Ladder
DNA size markers are widely used to estimate the size of DNA samples on agarose or polyacrylamide gelelectrophoresis (PAGE). DNA markers can be prepared by mixing PCR products with definite sizes.Alternatively, they are prepared by restriction enzyme digestion of the genomic DNA of bacteriophages ornatural and synthetic DNA plasmids. The present study describes engineering of ...
متن کاملHow to Select a Specified Area for Research?
سخن سردبیر Editorial مجله دانشگاه علوم پزشکی رفسنجان دوره 22، فروردین 1402، 2-1 چگونه یک حوزه مشخص برای پژوهش را انتخاب نمائیم؟ How to Select a Specified Area for Research? محسن رضائیان[1] Mohsen Rezaeian برای محققین جوان، انتخاب یک حوزه مشخص برای انجام پژوهش‌های‌شان بسیار ضروری است. پژوهش‌گران جوان به جای آن که توان و نیروی خود را مصروف موضوعات پژوهشی متفاوتی نمایند، با...
متن کاملThe influence of neighboring base pairs upon base-pair substitution mutation rates.
The 2-aminopurine-induced transition, A.T --> G.C, was studied at particular sites in bacteriophage T4 as a function of the nearby base-pair composition of the DNA. Changing a base pair changed the transition rate at the adjacent base pair up to 23-fold, and at the next base pair by a lesser amount. Destabilization was achieved by replacing an A.T base pair by a G.C base pair.
متن کاملA pair-to-pair amino acids substitution matrix and its applications for protein structure prediction.
We present a new structurally derived pair-to-pair substitution matrix (P2PMAT). This matrix is constructed from a very large amount of integrated high quality multiple sequence alignments (Blocks) and protein structures. It evaluates the likelihoods of all 160,000 pair-to-pair substitutions. P2PMAT matrix implicitly accounts for evolutionary conservation, correlated mutations, and residue-resi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1983
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/11.12.4229